Description

There was significant increase in peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) activities in diseased leaves of all three varieties-RGC-936 (susceptible), RGC-1003 (moderately resistant) and RGC-986 (resistant) of clusterbean. Significant decrease in catalase activity was observed in diseased leaves than healthy ones and this decrease was more pronounced in susceptible variety RGC-936.The pathogen related enzymes polygalacturonase trans-eliminase (PGTE), pectin trans-eliminase (PME), polygalacturonase (PG) and cellulolytic (Cx) enzymes activities were found lower at initial stages of observations.The soluble protein content in healthy leaves of RGC-986 was highest followed by RGC-1003 while, it was lowest in RGC-936. It was significantly decreased in diseased leaves of all three varieties. Maximum decrease in soluble protein due to infection was observed in RGC-936 followed by RGC- 986 and RGC-1003.Total phenolic contents were higher in healthy leaves of RGC-986 followed by RGC-1003 and RGC-936.