Description

Enzymatic saccharification and fermentation study was conducted in two steps to produce bio-ethanol from pea hulls and banana peels. In second effect of fermentation on pH, reducing sugar and bio-ethanol estimation was studied. The parameters considered in first step were incubation temperature (40°C) cellulose enzyme concentration (0.06ml/g slurry), time of treatment (48 h) and temperature (40°C). Inoculation level (4, 6, 8, 10 and 12 %), pH (4) and Time (36, and 48h) were used during fermentation. All the independent variables i.e. fermentation time, inoculation level and slurry ratio had significant effect on bio-ethanol yield. The optimum values of independent variables i.e inoculation time, inoculation level and slurry ratio to obtain minimum pH values are 43.6598 hr, 5.2675% and 47.2184% respectively To obtain maximum bio-ethanol yield the optimum values of inoculation time, inoculation level and slurry ratio are -80.892 hr, 10.94% and 633.7211% respectively. The optimum values of independent variables i. e inoculation time, inoculation level and slurry ratio to obtain minimum reducing sugar are 57.6462 hr, 9.9173% and -79.7462% respectively.