Description

In this study we have collected 21 patient samples having pyogenic skin infection to assess their antibiotic susceptibility by disc diffusion method and to isolate quinolone-resistant strains.Among 21 patient samples only four samples were quinolone-susceptible. A few of the quinolone-resistant samples are also methicillin resistant. The majority of the clinical isolates were gram-positive.8 samples within the 17 clinical isolates gave positive result in plasmid isolation.1 quinolone-susceptible but methicillin resistant strain also yielded plasmids. These 9 samples also gave distinct band pattern when they were digested with restriction enzyme EcoRI, HindIII, HinfI. Those isolated plasmids were transformed to E. coli DH5 strain,among the 8 samples 6 samples transformed quinolone resistance to sensitive cells.